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Mini-Circuits
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OriGene
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OriGene
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OriGene
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Miltenyi Biotec
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Alomone Labs
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Kingfisher Biotech
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Image Search Results
Journal: Journal of Bacteriology
Article Title: UpaG, a New Member of the Trimeric Autotransporter Family of Adhesins in Uropathogenic Escherichia coli
doi: 10.1128/jb.00122-08
Figure Lengend Snippet: FIG. 5. Adhesive properties of UpaG. (A) Adherence of CFT073, CFT073 upaG, and CFT073 PcL upaG strains to T24, HeLa, and BSC1 epithelial cells. Values correspond to the means standard deviations from three independent experiments (, denotes a P of 0.01). WT, wild type. (B) Adherence of CFT073 upaG and CFT073 PcL upaG cells to a mixed monolayer of T24 cells expressing the red fluorescence protein (red cells) and HeLa cells. Actin was labeled by phalloidin Alexa 647 (in blue), revealing all eukaryotic cells. Bacteria were observed using phase-contrast microscopy and colored green using Adobe Illustrator CS software. CFT073 PcL upaG adhered in significantly greater numbers to T24 bladder epithelial cells than to HeLa epithelial cells (left panel). In contrast, CFT073 upaG showed no difference in adherence to T24 bladder epithelial cells and HeLa epithelial cells (right panel).
Article Snippet: HeLa epithelial cells (ATCC number CCL-2), T24 cells (Marinpharm), T24-red epithelial cells (Marinpharm), and
Techniques: Adhesive, Expressing, Labeling, Bacteria, Microscopy, Software